Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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Centrifugar el tubo a g durante min a temperatura ambiente y descartar el sobrenadante con una pipeta. Further work is needed to evaluate their resistance level to Phytophthora infestans Mont. Chromosome analysis The numbers of chromosome of S. Protoplasts fusion and plant regeneration.

Fusion de protoplastos pdf

After that, colonies were dispersed into plates with solid MS13K regeneration medium Benke, An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L.

Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L. This technique is a tool for somatic hybrid characterization Baird et al.

In conclusion, somatic fusions between the cultivated potato Solanum tuberosum and the wild species S.


Protoplasts fusion from S. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: Somatoclonal variation in potato: Please recommend JoVE to your librarian. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers.

Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium. Irregular meiosis protplastos a somatic hybrid between S. The same primer amplified four specific bands of, and bp in S. Origins of cultivated potatoes and species relationships. Soja Glycine max L. Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. These bands also appeared in the fusion regenerants Figs.

Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed.

Final concentration was protoplasts per millilitre. Interspecific somatic hybrids between wild potato Solanum acaule Bitt. Due to that, fused and unfused protoplasts were cultured together.

Non-transgenic applications of plant tissue culture in potato.

Cultivo y fusion de protoplastos

A slot-blot hybridization method for screening somatic hybrids. Materials and methods Plant material and protoplast isolation. When colonies developed into microcalli, the alginate beads were depolimerized. To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis.


Centrifugar el tubo a x g por min a temperatura ambiente para cosechar protoplastos. These samples were obtained from rooted plants maintained in a greenhouse. A subscription to J o VE is required to view this article. This value is different from that obtained by Mattheij et al.

The origin of this chromosome instability has been studied elsewhere in potato Kumar, Skip to content Biology. In Vitro Cell Dev.

Cultivo y fusion de protoplastos [1990]

Nineteen plants were regenerated from calli Fig. The numbers of chromosome of S.

Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but little aneuploidy; on prrotoplastos other hand, regenerated plants from tetraploid genotypes produce a wide range of aneuploidy Kumar, The wild species S. We recommend downloading the newest fusio of Flash here, but we support all versions 10 and above. If the problem continues, please let us know and we’ll try to help. Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S.